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KMID : 0894520070110030227
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Development & Reproduction
2007 Volume.11 No. 3 p.227 ~ p.233
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Milt Properties and Spermatozoa Structure of Filefish(Thamnaconus modestus)
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Le Minh-Hoang
Lim Han-Kyu
Min Byung-Hwa
Kim Sung-Yeon
Chang Young-Jin
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Abstract
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The milt properties of filefish(Thamnaconus modestus) included physical properties of sperm and biochemical properties of seminal plasma. The physical properties of milt were 0.3±0.1 mL·fish－1 in sperm volume, 2.6±0.1×10? spermatozoa·mL－1 in sperm concentration and 73.3±6.7 in spermatocrit. The biochemical properties of seminal plasma contained 9.8±0.9 m㏖·L－1 potassium, 164.0±4.0 m㏖·L－1 sodium, 151.0±1.2 m㏖·L－1 chloride, 14.9±0.6 ㎎·dL－1 calcium, 7.2±0.1 ㎎·dL－1 magnesium, 1.0 ㎎·dL－1 glucose, 0.1 g·dL－1 total protein and 1.0 ㎎·dL－1 total lipid. The osmolality and pH of seminal plasma were 322.8±2.8 mOs㏖·㎏－1 and 7.7±0.1, respectively. The spermatozoon of filefish consisted of three parts: head without acrosome, mid-piece with five mitochondria and flagellum with “9+2” pattern. The head of spermatozoon in longitudinal section was horseshoe-shaped, and 1.3～1.6 ㎛ long and 1.0～1.3 ㎛ wide.
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KEYWORD
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Filefish, Thamnaconus modestus, Milt properties, Seminal plasma, Spermatozoa, Structure
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